Recombinant Human CASP2, N-His

Description of Recombinant Human CASP2, N-His

Introduction to Recombinant Human CASP2, N-His

Recombinant Human CASP2, N-His is a protein that plays a crucial role in the process of programmed cell death, also known as apoptosis. It is a member of the caspase family of proteins, which are enzymes involved in the regulation of apoptosis. This particular form of CASP2 is produced using recombinant DNA technology, making it a valuable tool in the study of apoptosis and potential drug target for various diseases.

Structure of Recombinant Human CASP2, N-His

The structure of Recombinant Human CASP2, N-His is composed of 303 amino acids, with a molecular weight of approximately 35 kDa. It contains a catalytic domain, which is responsible for its enzymatic activity, and a prodomain, which regulates its activation. The protein also has a histidine tag, or N-His, which is used for purification purposes.

The catalytic domain of Recombinant Human CASP2, N-His is highly conserved among caspase family members and contains a catalytic cysteine residue that is essential for its activity. The prodomain, on the other hand, is unique to CASP2 and is responsible for its regulation. It contains a caspase recruitment domain (CARD), which is involved in protein-protein interactions and activation of the enzyme.

Activity of this protein

Recombinant Human CASP2, N-His is an enzyme that plays a critical role in the process of apoptosis. It is activated in response to various stimuli, such as DNA damage, oxidative stress, and viral infection. Once activated, the prodomain is cleaved, and the catalytic domain is released, allowing it to cleave specific target proteins involved in the apoptotic pathway.

One of the main targets of Recombinant Human CASP2, N-His is poly (ADP-ribose) polymerase (PARP), a protein involved in DNA repair. By cleaving PARP, CASP2 initiates a cascade of events that ultimately leads to cell death. This process is crucial in maintaining the balance between cell survival and death, and dysregulation of CASP2 activity has been linked to various diseases, including cancer and neurodegenerative disorders.

Application of Recombinant Human CASP2, N-His

Recombinant Human CASP2, N-His has various applications in both research and potential drug development. Its role in apoptosis makes it a valuable tool for studying the mechanisms of cell death and its involvement in diseases. It can also be used to screen potential drugs that target the apoptotic pathway and regulate CASP2 activity.

Additionally, Recombinant Human CASP2, N-His has shown promising results as a potential drug target for various diseases. In cancer, for example, it has been found to be overexpressed in certain types of tumors, making it a potential target for cancer therapy. In neurodegenerative disorders, CASP2 has been implicated in the death of neurons, and inhibiting its activity may have therapeutic benefits.

Conclusion

In summary, Recombinant Human CASP2, N-His is a crucial protein involved in the process of apoptosis. Its structure, activity, and potential applications make it a valuable tool in the study of cell death and a potential drug target for various diseases. Further research on this protein may lead to the development of novel therapies for conditions such as cancer and neurodegenerative disorders.