Recombinant Human CEP97 Protein, N-His

Introduction

Recombinant Human CEP97 Protein, also known as centrosomal protein 97, is a highly conserved protein that plays a critical role in cell division and centrosome function. It is encoded by the CEP97 gene and is a key component of the centrosome, a cellular organelle responsible for organizing the microtubule network during cell division.

Structure of Recombinant Human CEP97 Protein

The recombinant form of CEP97 protein is produced through genetic engineering techniques, where the CEP97 gene is inserted into a suitable expression vector and then expressed in a host cell. The resulting protein is identical to the natural form of CEP97, with a molecular weight of approximately 97 kDa.

The structure of CEP97 protein is characterized by a coiled-coil domain at the N-terminus and a C-terminal domain containing a conserved WD40 repeat motif. This unique structure allows CEP97 to interact with various proteins, including other centrosomal proteins, to regulate centrosome function.

Function of Recombinant Human CEP97 Protein

CEP97 is a crucial component of the centrosome, where it plays a critical role in centrosome duplication and maturation. During cell division, the centrosome duplicates to form two new centrosomes, which are essential for organizing the microtubule network and ensuring proper cell division.

CEP97 is involved in the recruitment and assembly of other centrosomal proteins, such as CEP152 and CEP192, to the centrosome. It also regulates the activity of these proteins, ensuring proper centrosome function. Additionally, CEP97 is required for the maintenance of the structural integrity of the centrosome.

Application of Recombinant Human CEP97 Protein

Due to its critical role in cell division and centrosome function, recombinant human CEP97 protein has various applications in scientific research and drug development.

One of the main applications of CEP97 protein is in the study of centrosome biology and cell division. By studying the function and interactions of CEP97 with other centrosomal proteins, researchers can gain a better understanding of the mechanisms involved in centrosome duplication and maturation. This knowledge can also help in identifying potential targets for therapeutic intervention in diseases related to centrosome dysfunction, such as cancer.

Recombinant CEP97 protein can also be used in drug development, particularly in the development of anti-cancer drugs. As CEP97 is overexpressed in many types of cancer, targeting this protein could potentially inhibit cancer cell growth and proliferation. Additionally, CEP97 has been found to be essential for the survival of cancer cells, making it a promising target for cancer therapy.

Conclusion

In summary, recombinant human CEP97 protein is a crucial component of the centrosome, playing a critical role in cell division and centrosome function. Its unique structure and interactions with other centrosomal proteins make it an important target for scientific research and drug development. With its various applications, CEP97 protein continues to be a valuable tool in advancing our understanding of cell biology and in the development of new therapies for diseases related to centrosome dysfunction.