Recombinant Human ARHGAP10 Protein, N-His

Description of Recombinant Human ARHGAP10 Protein, N-His

Recombinant Human ARHGAP10 Protein: A Structural Overview

Recombinant Human ARHGAP10 Protein, also known as Rho GTPase-activating protein 10, is a human protein that plays a crucial role in regulating cell migration and adhesion. This protein is encoded by the ARHGAP10 gene and is a member of the RhoGAP family of proteins. In this article, we will provide a detailed description of the structure, activity, and applications of Recombinant Human ARHGAP10 Protein.

Structure of Recombinant Human ARHGAP10 Protein

The structure of Recombinant Human ARHGAP10 Protein consists of 1035 amino acids with a molecular weight of approximately 120 kDa. It contains a RhoGAP domain, a PH domain, and a C-terminal SH3 domain. The RhoGAP domain is responsible for the GTPase-activating activity of the protein, while the PH domain is involved in binding to phospholipids. The SH3 domain is responsible for protein-protein interactions.

The RhoGAP domain of Recombinant Human ARHGAP10 Protein has a conserved arginine finger motif, which is essential for its GTPase-activating activity. This domain interacts with the GTP-bound form of Rho proteins and stimulates their GTPase activity, leading to the conversion of active Rho-GTP to inactive Rho-GDP. This process plays a crucial role in regulating the activity of Rho proteins, which are involved in various cellular processes, including cell migration, adhesion, and cytoskeletal organization.

Activity of Recombinant Human ARHGAP10 Protein

Recombinant Human ARHGAP10 Protein has been shown to play a crucial role in regulating cell migration and adhesion. It acts as a negative regulator of Rho proteins, which are known to promote cell migration and adhesion. By stimulating the GTPase activity of Rho proteins, Recombinant Human ARHGAP10 Protein inhibits their activity, leading to the suppression of cell migration and adhesion.

In addition to its role in regulating Rho proteins, Recombinant Human ARHGAP10 Protein has also been shown to interact with other proteins involved in cell migration and adhesion. For example, it has been reported to interact with focal adhesion kinase (FAK) and p130Cas, which are key regulators of cell adhesion and migration. This interaction is thought to play a role in the regulation of focal adhesion turnover, which is crucial for cell migration.

Applications of Recombinant Human ARHGAP10 Protein

Recombinant Human ARHGAP10 Protein has been widely used in various research studies to investigate its role in cell migration and adhesion. It has also been used to study the regulation of Rho proteins and their downstream signaling pathways. In addition, Recombinant Human ARHGAP10 Protein has been used in cell-based assays to screen for potential inhibitors of its activity, which could have therapeutic potential for diseases involving abnormal cell migration, such as cancer.

Furthermore, Recombinant Human ARHGAP10 Protein has been used in structural studies to investigate its interaction with other proteins involved in cell migration and adhesion. These studies have provided valuable insights into the mechanism of action of this protein and its role in regulating cellular processes.

In conclusion, Recombinant Human ARHGAP10 Protein is a crucial protein involved in regulating cell migration and adhesion. Its structure, activity, and applications have been extensively studied, and it continues to be a subject of interest for researchers in the field of cell biology. With its potential therapeutic applications, Recombinant Human ARHGAP10 Protein holds great promise for the development of novel treatments for diseases involving abnormal cell migration and adhesion.