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E.coli
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Anti-GPCR
Anti-Chemokines & chemokine receptors
Anti-Spike & Anti-RBD
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IP guaranteed monoclonal antibody production
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IP guaranteed monoclonal antibody production
Monoclonal antibody production package details
Package content
Guarantees and price
Deliverables
Lead time
Antigen design (design of the most appropriate fragment to be produced)
Two gene syntheses (each gene optimized for a specific expression system) and two subclonings
Expression tests in two systems taken from
E. coli
,
B. subtilis
,
P. pastoris
, insect cells, and mammalian cells. To be determined by ProteoGenix. GST-tagged version also done in parallel for IP experiments
Production of the antigen in the most appropriate expression system depending on the yield and the project requirements.
Mice injections (5 mice)
Immune response control by IP
Selection of the best two mice for two fusions in order to have a large number and variety of clones
Screening of the fusion product by IP
Subcloning of two parental clones (2-3 rounds of limiting dilutions)
Production and purification of 2mg from the best clone
Level 1 : If we don’t succeed in producing an antibody that detects the immunogen by IP, we charge EUR 1 000
Level 2 : If we succeed in producing an antibody that works using IP against the immunogen, we charge a part of the cost
Level 3 : If after testing the purified antibody the customer wants to receive the hybridoma, we charge the full package price
10 -20 best clones at parental stage for customer’s selection
100 µg of purified antibody to confirm hybridoma purchase
50 µg of purified protein
After total payment:
1.9 mg of purified antibody
One hybridoma cell line (four frozen vials)
150 µg of purified protein
Gene in pUC57
Gene synthesis: 3-4 weeks
Protein production : 7-9 weeks
Injections: 8-12 weeks
Fusion & screening:5-6 weeks
Subcloning: 8-12 weeks
Antibody production:3-4 weeks
Total: 34-47 weeks
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Our application guaranteed monoclonal antibody production packages
Standard ELISA: ATX-PACK1M
Premium ELISA: ATX-PACK2M
Premium WB: ATX-PACK3M
Premium Flow Cytometry: ATX-PACK4M
Premium sandwich ELISA: ATX-PACK5M
Premium IF: ATX-PACK6M
Peptide: ATX-PACK8M
Modification specific: ATX-PACK9M
Premium IHC: ATX-PACK10M