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Recombinant Human CD3E Protein, N-His(cat. No.ARO-P10225) at 0.5µg/mL (100µL/well) can bind Human CD3E (OKT3) Monoclonal Antibody in indirect ELISA with Goat secondary antibody measured by OD450.
Flow-cytometry using anti-human CD3E antibody. Human Jurkat cell line were stained with an irrelevant antibody (Green Histogram) or an anti-human CD3E antibody monoclonal antibody (Catalog # ARO-A15835 , Red Histogram) at a concentration of 20 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a CoraLite488 conjugated goat anti-mouse antibody (Catalog # PTX17816) and cells analysed on a NovoCyte Flow Cytometer.
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PTX17816
PTX17818
ARO-P10225
PTX20654
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