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Olaratumab Biosimilar - Anti-PDGF-R alpha mAb - Research Grade can bind to its target in indirect ELISA with Goat secondary antibody measured by OD450.
Target Cells were stained with an irrelevant antibody or Olaratumab Biosimilar - Anti-PDGF-R alpha mAb - Research Grade at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Human IgG Polyclonal Antibody, FITC (PTX18862) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using PE anti-human CD140a antibody. MG-63 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD140a monoclonal antibody (Catalog: PX-TA1025, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using anti-human PDGFRA antibody. MG-63 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human PDGFRA monoclonal antibody (Catalog PX-TA1025, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: PTX18862) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using FITC anti-human CD140a antibody. MG-63 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD140a monoclonal antibody (Catalog: PX-TA1025, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Olaratumab Biosimilar - Anti-PDGF-R alpha mAb - Research Grade, on SDS-PAGE. The gel was stained overnight with Coomassie Blue. The purity of the antibody is greater than 95%.
KPTX193
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