Hydrophobic Interaction Chromatography (HIC) is a protein purification technique based on the protein’s degree of hydrophobicity and hydrophobic interaction with immobilized ligands. In this section, you will find a detailed description of HIC chromatography. This is not a stand-alone service. All our protein purification techniques are included in our Protein Expression Services
Each protein expression project is different, so it is of great importance to establish an adapted protein expression strategy well suited to your project needs. In this matter, the purification step is a determining factor to achieve your goals. Here are some key characteristics to help you define if this purification technique is suitable for your protein project :
Hydrophobic Interaction Chromatography is a protein separation technique. It is based on interactions between the hydrophobic region on the surface of proteins and hydrophobic ligands immobilized in the resin. The mechanism of action relies on the equilibrium between adsorption and desorption in the presence or not of salt. The higher the salt concentration is, the higher the protein-ligand interactions are.
HIC separation has both a stationary phase and a mobile phase:
Like other chromatography techniques, HIC is also composed of 3 major steps:
Hydrophobic interaction chromatography is a versatile separation tool. It can be used in many application areas:
To accomplish protein purification with HIC and achieve the awaited goals, it is of great importance to select the proper parameters for this purification. Here are some of the factors to consider before applying hydrophobic interaction chromatography:
In this context, there are two parameters that can influence protein separation:
To sum up, hydrophobic interaction chromatography is a versatile tool for protein purification. Based on the hydrophobic interactions created between proteins and ligands, HIC allows protein separation. This technique can be combined with other chromatography methods to concentrate the final product and obtain high resolutions.
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