Recombinant Human ALDOB, N-His

Description of Recombinant Human ALDOB, N-His

Introduction

Recombinant Human ALDOB, N-His is a protein that plays a crucial role in the metabolism of fructose. It is a modified form of the human ALDOB gene, which encodes for the enzyme aldolase B. This protein has been extensively studied and has shown promising results as a potential drug target for various metabolic disorders. In this article, we will discuss the structure, activity, and potential applications of Recombinant Human ALDOB, N-His.

Structure of Recombinant Human ALDOB, N-His

The structure of Recombinant Human ALDOB, N-His is similar to that of the native human aldolase B protein. It is a tetrameric enzyme, consisting of four identical subunits, with each subunit having a molecular weight of approximately 40 kDa. The protein has a globular structure and is composed of 363 amino acids.

The N-His tag in the name of the protein refers to the addition of a short sequence of six histidine residues at the N-terminus of the protein. This tag is commonly used in recombinant proteins to facilitate purification and detection. The addition of the N-His tag does not affect the structure or activity of the protein, making it a popular choice for recombinant protein production.

Activity of this protein

The primary function of Recombinant Human ALDOB, N-His is to catalyze the conversion of fructose-1-phosphate into glyceraldehyde and dihydroxyacetone phosphate. This reaction is essential for the metabolism of fructose, a simple sugar found in fruits and honey. Defects in the ALDOB gene can lead to hereditary fructose intolerance, a rare metabolic disorder characterized by the inability to metabolize fructose. Recombinant Human ALDOB, N-His has shown promising results in correcting this enzyme deficiency and restoring normal fructose metabolism in preclinical studies.

In addition to its role in fructose metabolism, Recombinant Human ALDOB, N-His has also been found to have anti-inflammatory properties. It has been shown to inhibit the production of pro-inflammatory cytokines and reduce the expression of adhesion molecules in immune cells. This makes it a potential therapeutic agent for inflammatory conditions such as rheumatoid arthritis and inflammatory bowel disease.

Applications of Recombinant Human ALDOB, N-His

The most significant application of Recombinant Human ALDOB, N-His is in the treatment of hereditary fructose intolerance. Preclinical studies have shown that this protein can effectively correct the enzyme deficiency and restore normal fructose metabolism in animal models. Clinical trials are currently underway to evaluate the safety and efficacy of Recombinant Human ALDOB, N-His in humans with this rare disorder.

In addition to its potential as a therapeutic agent, Recombinant Human ALDOB, N-His also has diagnostic applications. The N-His tag attached to the protein allows for easy purification and detection, making it a valuable tool for researchers studying fructose metabolism and related disorders.

Conclusion

Recombinant Human ALDOB, N-His is a modified form of the human aldolase B protein with a crucial role in fructose metabolism. Its structure is similar to the native protein, and the addition of the N-His tag does not affect its activity. This protein has shown promising results as a potential drug target for hereditary fructose intolerance and has potential diagnostic applications. Further research and clinical trials are needed to fully explore the therapeutic potential of Recombinant Human ALDOB, N-His in treating metabolic disorders and inflammatory conditions.