Recombinant Human GADD45A, N-His

Description of Recombinant Human GADD45A, N-His

Introduction to Recombinant Human GADD45A, N-His

Recombinant Human GADD45A, N-His is a protein that plays a crucial role in cellular stress response and DNA damage repair. It is a member of the Growth Arrest and DNA Damage-inducible (GADD) family of proteins and is encoded by the GADD45A gene. The recombinant form of this protein, also known as rhGADD45A, N-His, is produced through genetic engineering techniques and has various applications in the field of drug development and research.

Structure of Recombinant Human GADD45A, N-His

The recombinant form of GADD45A, N-His is a 165 amino acid protein with a molecular weight of approximately 18.5 kDa. It contains a hexahistidine (N-His) tag at the N-terminus, which allows for easy purification and detection of the protein. The crystal structure of rhGADD45A, N-His has been determined, revealing a compact globular structure with three alpha helices and two beta sheets. This structure is similar to other members of the GADD family, such as GADD45B and GADD45G.

Activity of this protein

Recombinant Human GADD45A, N-His is a stress-responsive protein that is induced in cells upon exposure to various stressors, such as DNA damage, oxidative stress, and hypoxia. It functions as a molecular sensor and transducer, relaying signals from the stress stimuli to downstream signaling pathways. rhGADD45A, N-His has been shown to interact with multiple proteins involved in cell cycle regulation, DNA repair, and apoptosis, suggesting its role in maintaining genomic stability and cell survival.

One of the key functions of rhGADD45A, N-His is its involvement in DNA damage repair. It has been demonstrated to interact with and activate the p38/MAPK signaling pathway, which is crucial for the repair of DNA damage. Additionally, rhGADD45A, N-His has been shown to enhance the activity of the tumor suppressor protein p53, which plays a critical role in DNA repair and cell cycle control. This suggests that rhGADD45A, N-His may have potential therapeutic applications in cancer treatment.

Application of Recombinant Human GADD45A, N-His

Due to its important role in cellular stress response and DNA damage repair, rhGADD45A, N-His has been extensively studied for its potential applications in drug development and research. One of the main applications of this protein is as a drug target for cancer therapy. The dysregulation of GADD45A has been linked to various types of cancer, and the use of rhGADD45A, N-His as a therapeutic agent may help in restoring its normal function and inhibiting tumor growth.

Moreover, rhGADD45A, N-His has also been used as a research tool to study the mechanisms of cellular stress response and DNA damage repair. Its recombinant form allows for easy purification and detection, making it a valuable tool for studying protein-protein interactions and signaling pathways involved in these processes. Additionally, rhGADD45A, N-His has been used in in vitro and in vivo experiments to investigate its potential role in other diseases, such as neurodegenerative disorders and cardiovascular diseases.

Conclusion

Recombinant Human GADD45A, N-His is a stress-responsive protein with important functions in cellular stress response and DNA damage repair. Its recombinant form has various applications in drug development and research, particularly in the field of cancer therapy. Further studies on this protein may provide insights into its potential as a therapeutic target for various diseases and contribute to the development of novel treatments.