Recombinant Human KRT15, N-His

Description of Recombinant Human KRT15, N-His

Introduction to Recombinant Human KRT15, N-His

Recombinant Human KRT15, N-His is a protein that plays a crucial role in the structure and function of the skin. It is a member of the keratin family of proteins, which are the main structural components of hair, nails, and the outer layer of skin called the epidermis. KRT15, also known as Keratin 15, is produced by the KRT15 gene and is found primarily in the basal layer of the epidermis. The N-His tag is added to the recombinant protein during the production process to aid in purification and detection.

Structure of Recombinant Human KRT15, N-His

Recombinant Human KRT15 is a 52 kDa protein that consists of 460 amino acids. It has a unique structure, with a central alpha-helical rod domain flanked by non-helical head and tail domains. The N-His tag is located at the N-terminus of the protein and consists of six histidine residues. This tag allows for easy purification of the protein using affinity chromatography techniques.

Activity of this protein

Recombinant Human KRT15, N-His is involved in the formation and maintenance of the skin’s structural integrity. It forms a network with other keratin proteins, providing strength and stability to the epidermis. KRT15 also plays a role in cell signaling and communication, as well as cell migration and wound healing. Mutations in the KRT15 gene have been linked to skin disorders such as epidermolysis bullosa simplex and palmoplantar keratoderma.

Application of Recombinant Human KRT15, N-His

The unique structure and function of Recombinant Human KRT15, N-His make it a valuable tool in various research and clinical applications. Its ability to form a network with other keratin proteins makes it an important target for studying skin disorders and developing treatments. The addition of the N-His tag allows for easy purification and detection of the protein, making it useful for protein-protein interaction studies.

One potential application of Recombinant Human KRT15, N-His is in drug targeting. As KRT15 is highly expressed in the basal layer of the epidermis, it could be targeted by drugs for the treatment of skin disorders. Additionally, the N-His tag could be used as a target for drug delivery systems, allowing for targeted delivery of drugs to the skin.

Furthermore, Recombinant Human KRT15, N-His can also be used in the development of cosmetic products. As it plays a crucial role in maintaining skin integrity, it could be incorporated into skincare products to improve skin health and appearance. The N-His tag could also be used as a marker for the presence of the protein in these products.

In conclusion, Recombinant Human KRT15, N-His is a vital protein in the structure and function of the skin. Its unique structure and activity make it a valuable tool in various research and clinical applications, including drug targeting and cosmetic product development. With the addition of the N-His tag, this protein becomes even more versatile and useful in scientific studies. Further research on this protein could lead to new insights and treatments for skin disorders and other related conditions.